Current Research Projects
"Comprehensive Physicochemical Characterization of Nanocellulose Materials”. The major goal of this work is to detect and measure the nanometer-sized components within micro-fibrillated cellulose materials. Physicochemical characterization of the materials include microscopy, spectroscopy, spectrometry, and chromatography.
Investigator(s): Marina Mulenos George, Thelma Ameh, Desirae Carrassco
"Synthesis and characterization of nanometer sized liposomes for encapsulation and microRNA transfer to breast cancer cells”. The use of liposomes as a drug delivery carrier (DDC) for the treatment of various diseases, especially cancer, is rapidly increasing, requiring more stringent synthesis, formulation, and preservation techniques to bolster safety and efficacy. Liposomes, otherwise referred to as phospholipoid vesicles are self-assembled colloidal particles. When formed in either the micrometer or nanometer size range they are ideal candidates as DDC because of their biological availability, performance, activity, and compatibility. Defining and addressing the critical quality attributes (CQA) along the pharmaceutical production scale will enable a higher level of quality control for reproducibility. Data shows that microRNA can be loaded into nanometer-sized liposomes, preserved for months in a dried form, and maintain encapsulation after extended time periods in storage.
Investigator(s): Henry Lujan, Sahar Pradhan, Gaby Cruz, Desirae Carrassco
"Comparing the Basal-level Gene and Protein Expressions of Human Cell-Types”. Using cell culture-based models to measure the hazards of particle systems has become routine and derivative over the past few years. The research presented in this study analyzes the differences among cells retrieved from varying depths of the pulmonary system and in varying diseased states. There is a need to advance cell line use and suggest guidelines on which cells to use for specific experiments.
Investigator(s): Henry Lujan, Sahar Pradhan, London Steele, Irkan Notta
"Comprehensive Physicochemical Characterization of Nickel Colloid and Nickel Oxide Nanomaterials: Challenges and Opportunities for Environmental Health”. The objective of this study is to execute a comprehensive material characterization of four nickel nanomaterials, investigating the chemical nature (i.e. chemical composition, surface reactivity, and ion-to-particle ratios in aqueous suspensions) and physical properties (i.e. size and size distribution, morphology, and aggregation/agglomeration state).
Investigator(s): Marina Mulenos George, Desirae Carrassco, Daniel Kang
“Characterizing Nanoparticle–Protein Coronas: Using Analytical Methods to Detect and Identify Proteins Adsorbed onto Gold Particle Surfaces”. The use of nanomaterials in pharmaceutical research is increasing. Engineered nanomaterials have been proposed as drug-carriers and can deliver precise doses of specific drugs to targeted locations. However, upon entering blood stream, proteins readily adsorb onto and desorb from the surface of these drug products. This cloud of adsorbed proteins is known as the ‘protein corona’ and is postulated to critically alter either the drug product’s therapeutic (positive) or toxicological (negative) effects. In order to understand the effects related to the protein corona, detection and identification of the proteins which make up the corona is necessary. Data suggest that control over protein corona formation is dependent on particle surface chemistry may be predictable, and holds promise as an engineering technique to employ therapies.
Investigator(s): London Steele, Marina Mulenos George, Andreanna Burman
“Antimicrobial Encapsulated Nanoparticles Project”. Vector control has become a public health crisis for humans, animal wildlife, and plant health. While most vector eradication methods have focused on pathogens spread in human populations, pathogens that spread in plant populations are equally detrimental. Plant health is a central tenant in the agriculture industry. When a segment of agriculture is damaged by a disease or pathogen spread by an organism, the industry risks substantial economic and sustainability losses. The goal of this proposal is to develop a series of proto-typical Antimicrobial Encapsulated Nanoparticle Systems that demonstrate multi-functional properties in the delivery of antibacterial, pesticidal, and/or therapeutic activity to Asian citrus psyllid, CandidatusLiberibacterbacteria, and/or citrus trees, respectively.
Investigator(s): Thelma Ameh, Bailey Sharp
“Determining the biological mechanisms of action for environmental exposures: Applying CRISPR/Cas9 to toxicological assessments”. As the rapidly evolving field of nanotechnology continues to increase the number of products containing advanced materials, it is imperative that the field of toxicology maintains the same growth and adapt to properly guide public health safety recommendations. To date, the mechanisms of toxic action for most metal-based engineered nanomaterials are hypothesized as oxidative stress through the generation of reactive oxygen species (ROS). However, the exact mechanism of action or pathway through which toxicity is induced remains unknown. However, these mechanisms of toxicity can be uncovered by reviewing the techniques and validation studies undertaken by toxicologist in other fields. Specifically, the emergence of new laboratory techniques such as the CRISPR/Cas9 system for single gene knock-outs, CRISPR interference (CRISPRi), and CRISPR libraries, it is now possible to elucidate the exact mechanism through which metallic nanomaterials perturb normal cellular function.
Investigator(s): Henry Lujan, Sahar Pradhan
"Community Health on a Global Health Spectrum." This project reviews the current research in Global Health and describes a multi-faceted framework to understand and incentivize individuals to improve environmental health in their locality. We hypothesize that by tackling the misconceptions around greenhouse gas (GHG) emissions as it relates to community and individual health, involvement of community stakeholders will improve. It is hoped that the community can not only contribute to a creative solution to reduce GHG emissions, but to also educate and incentivize those individuals who are not traditionally included in the conversation.
Investigator(s): Easton Evans
"Investigating Traffic-Related Diesel Particulate Matter Exposure on the Basal Gene and Protein Expressions in Normal and Asthma-Derived Epithelial Lung Cells”. This research contributes to a deeper understanding of the toxicological relationships between human lung epithelial cells diseased state and exposure to diesel particulate matter. Individuals living near roadways may be at risk for detrimental airway malformation and exacerbated respiratory illnesses, such as asthma.
Investigator(s): Brendan Camp, Alicia Duval